FIELD: medicine, biochemistry. SUBSTANCE: set has buffer as 3-10 M urea solution, binding buffer as 2.5-5.0 M sodium chloride solution, eluting buffer as 10 mM Tris-HCl solution and 1 mM Trilon B at pH 8, 80-% ethyl alcohol solution as washing out buffer and silica gel as a sorbent. The use of this set ensures to isolate DNA from biological samples at high yield and purity degree that can be used in medicinal practice for diagnostic aims, in part, for diagnosis of hereditary, malignant and viral diseases. EFFECT: improved quality and enhanced effectiveness of the set proposed.
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Authors
Dates
1998-08-10—Published
1996-07-10—Filed