FIELD: medicine.
SUBSTANCE: kit consists of a lysing buffer based on 3-10 M urea solution, a binding buffer based on 2.5-5 M sodium chloride, an eluating buffer based on 10 mM Tris-HCl and 1 mM Trilon B at pH 8 and 80% ethanol as a washing buffer. As a sorbent, the kit comprises quartz sand having a particle size of 10-50 mcm.
EFFECT: kit enables the high-yield, high-purity and large molecular weight DNA recovery from biological objects.
2 dwg, 1 ex
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Authors
Dates
2015-01-10—Published
2013-11-11—Filed