METHOD OF PREPARING LEUZEA CARTHAMOIDES SOMATIC EMBRYOIDES IN CULTURE IN VITRO Russian patent published in 1999 - IPC

Abstract RU 2130709 C1

FIELD: biotechnology. SUBSTANCE: invention relates to preparing embryoides in plant tissue culture. Leusea carthamoides tissue culture is placed in nutrient medium in the presence of trace elements by Murasige-Skoog and macroelements by Sierlis, hormones, mg/l: 2,4-dichlorophenoxy-acetic acid 0.1-1; 6-benzylaminopurine 0.3-1.5; casein hydrolyzate 150-250 and vitamins: inositol 500-5000; thiamine hydrochloride 2-3; pyridoxine hydrochloride 1-2; niacin hydrochloride 1-2. Medium is sterilized and culture is cultured in scattering light at an additional illumination for 30-90 min by ultraviolet light. EFFECT: accelerated method of preparing. 3 ex

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RU 2 130 709 C1

Authors

Repjakh S.M.

Velichko N.A.

Jushkova E.V.

Nikonorova E.V.

Dates

1999-05-27Published

1997-07-25Filed