METHOD OF ISOLATION OF STRAIN-DESTRUCTORS EXHIBITING POLYSUBSTRATE SPECIFICITY Russian patent published in 1999 - IPC

Abstract RU 2133775 C1

FIELD: ecological microbiology. SUBSTANCE: microorganism sources exhibiting polysubstrate specificity with respect to toxic agents are mixed with semiliquid agarized synthetic minimal medium and stratified its as a second layer on a similar more solid medium. Separate paper disks impregnated with toxic agent solutions are placed on surface of solidified semiliquid agar. Plates are incubated at 28-30 C for a week at every day scanning. Microorganism clones which are able to utilize toxic agents form a visible growth as a ring around paper disks containing these toxic agents. Microorganism culture forming the ring growth around disks containing different toxic agents shows polysubstrate specificity. Clones selected from these colonies show the enhanced destructive activity. EFFECT: selection of the most valuable strains of microorganisms as active destructors of organic pollution of environment. 2 ex

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RU 2 133 775 C1

Authors

Denisov I.I.

Tikhonov N.G.

Iljukhin V.I.

Dates

1999-07-27—Published

1998-01-06—Filed