METHOD OF CLEANING PLASMA PROTEINS FROM LIPOPROTEINS (VARIANTS THEREOF) Russian patent published in 1999 - IPC

Abstract RU 2143267 C1

FIELD: medicine, more particularly, cleaning of plasma proteins from lipoproteins. SUBSTANCE: proteins are cleaned by treatment with chloroform in the presence of EDTA-Na or EDTA or buffers containing borate ions, succinate ions, nitrate ions and subsequent addition of calcium or magnesium salts to remove precipitate by centrifugation. In addition to 2- step removal of lipoproteins, also described is single- step variant of method which comprises treating plasma proteins with chloroform and subsequently adding soluble calcium or magnesium salts. The resulting precipitate is removed by centrifugation. EFFECT: higher degree of delipidization and higher yield. 7 cl, 4 ex, 1 tbl

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RU 2 143 267 C1

Authors

Israfilov A.G.

Ljutov A.G.

Kudasheva G.B.

Alsynbaev M.M.

Dates

1999-12-27—Published

1998-01-05—Filed