METHOD OF PREPARING STRAIN-PRODUCER OF FUSED PROTEIN APROINS FOR HUMAN INSULIN PREPARING Russian patent published in 2000 - IPC

FIELD: biotechnology, molecular biology, hormones. SUBSTANCE: method of development of the strain Escherichia coli - a producer of fused protein Aproin for human insulin preparing involves construction of complete proinsulin structural gene using chemical-pharmaceutical synthesis, modelling and measurement of optimal structure of IgG-binding domain of protein A, synthesis of the most optimal structure of lider peptide, its binding to form fused protein Aproin, transfer to expressing vector and transformation of E. coli cells. Method ensures to devise the most optimal structure of fused protein for human insulin preparing. EFFECT: improved method of strain-producer construction, simplified and safety technology. 3 cl, 9 dwg