FIELD: medicine, virology. SUBSTANCE: invention relates to effective and highly specific methods of assay of parvovirus B 19 in serum blood. Method is based on the use two-stage polymerase chain reaction involving preliminary heat treatment of analyzed sera at 95 C for 10 min. Method involves the use of sera not treated with enzyme proteinase K. At the first stage of reaction method involves the use pair at temperature annealing 44 C and consisting of the nucleotide sequences 5'-GACCTCCAAACCAC-3' and 5'-AATTGCTGATACACAG-3' and at the second stage - pair at temperature annealing 56 C consisting of the nucleotide sequences 5'-CAATTGTCACAGACACCAG-3' and 5'-TGCAATCCAGACAGGTAAG-3'. Two stages of reaction are carried out in a single tube. EFFECT: accelerated testing, decreased contamination of samples, decreased cost of analysis. 2 dwg
