FIELD: biotechnology, genetic engineering, molecular biology. SUBSTANCE: invention proposes highly specific primers ensuring to detect human herpes simplex-1 virus type-1 DNA for carrying out polymerase chain reaction from different clinical samples. Taken unique oligonucleotides do not show cross-reactions with related species that ensures to determine the presence or absence of human herpes simplex type-1 DNA in a single-step procedure of polymerase chain reaction carrying out rapidly and reliably. Invention can be used for diagnosis and identification of human herpes simplex type-1 virus and for the solution of scientific-research problems in investigations of viruses of this group. EFFECT: improved method of assay. 2 dwg, 2 ex
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