FIELD: medicine. SUBSTANCE: the innovation deals with the methods for detecting the quantity of viral genomes in a blood sample. The quantitative standard for evaluating the quantity of hepatitis C virus genomes is elaborated in patients' blood serum samples. It is, also, developed the method of quantitative evaluation of hepatitis C virus RNA in blood serum by using a retroviral vector containing a modified sequence of hepatitis C virus as an internal standard to be used for amplification in PCR, and a selective marker. The suggested retroviral standard is obtained from the culture of packing cells by simply gathering the cultivation medium where cells were growing. The presence of resistance gene to puromicin in a vector enables to detect the titer of standard's viral particles in experiments on infecting the cell cultures. The obtained viral particles of the standard are not pathogenic for people and animals. EFFECT: higher efficiency and accuracy of detection. 1 dwg
| Title |
Year |
Author |
Number |
| METHOD OF DETECTION OF HEPATITIS C VIRUS IN HUMAN SERUM BLOOD |
1998 |
- Ogrel' O.D.
- Sudarikov A.B.
|
RU2150505C1 |
| VIRAL PARTICLES CONTAINING ALPHAVIRUS VECTOR AND METHOD FOR PREPARING SAID VIRAL PARTICLE |
2004 |
|
RU2398875C2 |
| RECOMBINANT PLASMIDE DNA pVar15-HIV-LTR, CARRYING CLONED FRAGMENT GENOME HIV OF 1 TYPE FROM CONSERVATIVE SITE OF 5-LTR SEQUENCE, RECOMBINANT PLASMIDE DNA pBluKSM-HIV-LTR mod CARRYING CLONED MODIFIED FRAGMENT OF SAME SITE GENOME HIV OF 1 TYPE, TEST SET FOR QUANTITATIVE EXPRESS IDENTIFICATION OF GENOME HIV-1 OF ANY TYPE IN ASSAY AND METHOD WITH ITS USE |
2006 |
- Kostina Elena Viktorovna
- Sinjakov Aleksandr Nikolaevich
- Rjabinin Vladimir Alekseevich
|
RU2350650C2 |
| RECOMBINANT PLASMID DNA pGEM-Puro-DS-Apo COMPRISING SYNTHETIC APOPTIN GENE FLANKED BY SEQUENCES OF VACCINIA VIRUS GENOME OF ISLAND C10L-C12L, AND RECOMBINANT STRAIN VVdGF-ApoS24/2 OF VACCINIA VIRUS PRODUCING APOPTIN |
2012 |
- Chumakov Petr Mikhajlovich
- Kochneva Galina Vadimovna
- Babkina Irina Nikolaevna
- Lupan Tat'Jana Anatol'Evna
- Grazhdantseva Antonina Anatol'Evna
- Judin Pavel Valer'Evich
- Sivolobova Galina Filippovna
- Popov Evgenij Gennad'Evich
- Netesov Sergej Viktorovich
|
RU2492238C1 |
| IMPROVING GENETIC CONSTRUCTS FOR PROVIDING HIGH EFFICACY OF ANTI-HIV THERAPY |
2013 |
- Glazkova Dina Viktorovna
- Bogoslovskaja Elena Vladimirovna
- Markelov Mikhail Leonidovich
- Shipulin German Aleksandrovich
- Pokrovskij Valentin Ivanovich
- Pokrovskij Vadim Valentinovich
|
RU2533817C1 |
| PLASMIDS AND METHOD OF PRODUCING VIRUS PARTICLES |
2016 |
- Toro Ascuy, Daniela
- Tambley Zamorano, Carolina
- Spencer Ossa, Eugenio
- Cortez San Martin, Marcelo
- Beltran Pavez, Carolina
|
RU2708151C2 |
| METHOD OF PRODUCING MATERIAL WITH HIGH TITRE OF HEPATITIS E VIRUS AND ANALYSIS OF HEPATITIS E VIRUS TITRATION |
2016 |
- Buno Brett
- Journigan Terri
- Hotta Joann
- Burdick Michael
|
RU2691123C1 |
| NEW FULL-LENGTH GENOMIC RNA OF JAPANESE ENCEPHALITIS VIRUS, INFECTIVE cDNA JEV OBTAINED FROM THE SAME AND USES THEREOF |
2003 |
- Li Seung Khan
- Li Jang-Min
- Jun Sang-Im
|
RU2307872C2 |
| METHOD FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS IN BIOLOGICAL MATERIAL WITH A LOW VIRAL LOAD BASED ON TWO-STAGE PCR WITH DETECTION BY THREE TARGETS IN REAL TIME |
2020 |
- Serikova Elena Nikolaevna
- Semenov Aleksandr Vladimirovich
- Ostankova Yuliya Vladimirovna
- Totolyan Areg Artemovich
|
RU2763173C1 |
| METHODS AND REAGENT KITS FOR GENE-AMPLIFICATION DIAGNOSTICS INVOLVING PREPARATION AND RNA PREPARATION METHODS |
2000 |
- Fedorov N.A.
- Elov A.A.
- Sukhanov Ju.S.
|
RU2164532C1 |
