FIELD: biotechnology, genetic engineering. SUBSTANCE: invention relates to preparing highly specific primers ensuring to detect human cytomegalovirus DNA in carrying out polymerase chain reaction from different clinical samples. Taken unique oligonucleotides do not show crossed reactions with relative species that ensures to carry out a limited single-step procedure of polymerase chain reaction. Invention can be use for diagnostic aims in detection of cytomegaloviris and for scientific-research aims in investigation of cytomegaloviral infection. EFFECT: rapid and reliable method of assay of cytomegalovirus DNA presence. 1 tbl, 3 dwg, 2 ex
Authors
Dates
2001-04-10—Published
1999-12-07—Filed