POLYPEPTIDE AND METHOD OF ITS SYNTHESIS, REAGENT FOR IMMUNO-ANALYSIS, METHOD OF ASSAY OF ANTIBODY PRESENCE, METHOD OF INDUCTION OF IMMUNE RESPONSE Russian patent published in 2000 - IPC

FIELD: chemistry of peptides, immunology. SUBSTANCE: invention relates to polypeptides used as immunological reagents for assay, prophylaxis and treatment of infections induced by hepatitis C virus. Invention describes a new polypeptide including truncated sequence of hepatitis C virus and containing epitope of hepatitis C virus of the formula aa_x-aa_y. This polypeptide is able to induce immune response in humans and a means amino acid; x and y are whole numbers at condition that y - x ≥ 14; aa_x-aa_y means part of amino acid sequence in figure 1 and x is taken among the group: 66, 413, 465, 540, 1218, 1940, 2244, 2281. Indicated polypeptide can contain an optional sequence of N-terminal amino acids corresponding to the sequence of polypeptide of hepatitis C virus which adjoins to N-end of amino acid aa_x to which it bound or an optional sequence of C-terminal amino acids corresponding to the sequence of polypeptide of hepatitis C virus which adjoins to C-end of amino acid aa_y to which it bound. The total amount of additional amino acids does not exceed 15 for aa₄₁₃-aa₄₂₇, 4 for aa₄₆₅-aa₄₈₀, 85 for aa₅₄₀-aa₅₅₄, 10 for aa₂₂₄₄-a₂₂₅₈ and 30 for aa₂₂₈₁-aa₂₃₀₀. Invention describes method of assay of antibody presence and method of induction of immune response also. EFFECT: improved method of synthesis, new polypeptide indicated above. 6 cl, 2 tbl