FIELD: biotechnology, L-amino acids. SUBSTANCE: L-amino acids, such as L-phenylalanine or L-threonine are prepared by culturing microorganism Escherichia coli with enhanced ability for producing L-amino acids. Then amino acids are isolated from cultural fluid. Strains of microorganism Escherichia coli AJ 12739 (pAYCRER-YEDA2) and MG 442/pYEDA1 show the enhanced ability for producing L-phenylalanine and L-threonine, respectively. Invention provides preparing L-amino acids with high degree of effectiveness. EFFECT: improved preparing method. 7 cl, 3 tbl, 4 ex
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