FIELD: microbiology. SUBSTANCE: method involves culturing one or complex of Leptospira pathogenic strains, disintegration of bacterial mass by deep freezing of suspension followed by its extrusion, centrifugation, extraction of sediment with 2.5% sodium chloride solution, centrifugation and precipitation of the first and the second supernatants with ammonium sulfate solution. Invention can be used in biotechnology for preparing diagnostic preparations. EFFECT: increased specific activity of antigen, increased yield, decreased cost of ready product.
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