FIELD: medicine.
SUBSTANCE: invention refers to synthetic oligonucleotide primers for detecting 1a and 1b virus subgenotypes of viral diarrhoea - bovine mucosal disease (VD - BMD) and to a method of applying said synthetic oligonucleotide primers for detecting 1a and 1b virus subgenotypes of viral diarrhoea - bovine mucosal disease. The method can be used in veterinary virology for subgenotype differentiation of strains and isolates of genotype 1 virus. The offered method involves a PCR with pairs of primers SEQ ID NO:1 5'-tcgacgccttaacatgaaggt-3' and SEQ ID N0:3 5'-ccatgtgccatgtacag-3' for subgenotype la, SEQ ID NO:2 5'-tcgacgctttggaggacaagc-3' and SEQ ID NO:3 5'-ccatgtgccatgtacag-3' for subgenotype lb. It is followed with detecting a DNA fragment sized 186 bp in each reaction. Further, the presence in an analysed material of 1a and lb subgenotype virus of viral diarrhoea - bovine mucosal disease is concluded.
EFFECT: invention allows express subgenotype differentiation of genotype one VD - BMD virus strains.
2 cl, 2 dwg, 2 tbl, 4 ex
