FIELD: medicine; biotechnology.
SUBSTANCE: invention claims method of detecting animals persistently infected with germs of viral diarrhea, a mucosa disease of cattle. Method involves blood serum sampling from 20 animals from herd, newborn to 12 months old, separation of virus RNA from united samples, oligonucleotide primer synthesis, virus RNA amplification in polymer chain reaction, identification of polymer chain reaction products by electrophoresis. In case of positive reaction, fragment of 731 nucleotide couples is detected.
EFFECT: possible application in veterinary for infected animal detection.
3 cl, 5 tbl, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD OF EXPOSING RNA VIRUS OF MUCOSAL DISEASE IN MUCUS OF CATTLE USING SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION WITH SIMULTANEOUS DIFFERENTIATION OF VIRUS STRAINS TO 1 AND 2 GENOTYPES | 2007 |
|
RU2347812C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD FOR DETECTING RNA ATYPICAL PESTIVIRUS OF CATTLE | 2016 |
|
RU2607025C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR DETECTING 1A AND 1B VIRUS SUBGENOTYPES OF VIRAL DIARRHOEA - BOVINE MUCOSAL DISEASE AND METHOD OF APPLYING THEREOF | 2009 |
|
RU2409673C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD OF BOVINE RESPIRATORY SYNCYTIAL INFECTION RNA VIRUS DETECTION BY SYNTHETIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION (PCR) | 2009 |
|
RU2405039C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES AND METHOD FOR DETECTING BOVINE PESTIVIRUS H | 2019 |
|
RU2728342C1 |
| METHOD OF DETECTION OF PIG CLASSIC PLAGUE VIRUS | 1997 |
|
RU2120994C1 |
| METHOD OF DETECTION OF CATTLE VIRAL DIARRHEA VIRUS (MUCOSA DISEASE) USING SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION | 1999 |
|
RU2158306C2 |
| KIT FOR DIFFERENTIATING CATTLE PESTIVIRUSES AND METHOD FOR DIFFERENTIATING CATTLE PESTIVIRUSES | 2019 |
|
RU2731716C1 |
| OLIGONUCLEOTIDE PRIMERS FOR DETECTING BOVINE ENZOOTIC LEUKAEMIA VIRUS BY POLYMERASE CHAIN REACTION | 2024 |
|
RU2824666C1 |
| METHOD OF DETECTING PATHOGENS OF RESPIRATORY INFECTIONS IN CATTLE: BPIV, BRSV, BHV-4, BCoV, BVDV-1, BVDV-2, BVDV-3 BASED ON MULTIPLEX POLYMERASE CHAIN REACTION (PCR) | 2022 |
|
RU2798286C1 |
