METHOD OF ASSAY OF DEFICIENCY OF ISOTYPES OF HUMAN COMPLEMENT COMPONENT C4A AND C4B Russian patent published in 2001 - IPC

FIELD: medicinal immunology. SUBSTANCE: invention relates to methods of assay of isotypic composition of complement components in human serum blood in diagnosis of some diseases and in biological preparations. Method A: firstly, in micropanel wells native or aggregated by preliminary heating immunoglobulins of class G or M are sorbed successively for preferred determination of activity of C4A. Method B: or cellular wall lipopolysaccharides of gram-negative bacteria are sorbed for preferred determination of activity C4B. Then component C4 to be analyzed is added into wells taken from solutions containing its in the presence of guinea pig serum. Amount of covalently bound human complement component C4 as a result of activation is determined by product of enzymatic reaction using antibodies to component C4 of human complement, namely, to fraction of immunoglobulins G covalently bound with enzyme. Ratio of activity obtained by method A and B is measured. If these ratios are above or lower by 2-fold or more as compared with standard value obtained for serum pool from 10 healthy donors then deficiency of C4A or C4B occurs, respectively. Method ensures to determine deficiency of isotypes of human complement component C4A and C4B based on immunoenzymatic assay and can be used in clinical practice. EFFECT: improved method of assay. 1 tbl, 1 ex