FIELD: medicinal immunology.
SUBSTANCE: the present innovation deals with detecting functional activity of complement components in human blood serum while predicting the row of diseases and in biological preparations, as well. One should sorb immunochemically pure immunoglobulin G in microplot's holes, then it is necessary to supplement the holes with an analyzed sample that contains human complement C3 component at unknown activity, and solution that contains guinea-pig's serum to carry out incubation. Then the content of holes should be poured out to introduce enzymatic conjugate with antibodies against human C3 component and substrate of this enzyme into the holes. C3 activity should be calculated according to the quantity of developed product of enzymatic reaction.
EFFECT: higher sensitivity and shortened terms of detection.
2 dwg, 2 ex
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