METHOD FOR DIAGNOSING INFECTION BY DETECTING GENETIC MATERIAL OF PATHOGEN IN SAMPLE UNDER STUDY Russian patent published in 1999 - IPC

Abstract RU 2143113 C1

FIELD: medicine. SUBSTANCE: method involves duplicating DNA or RNA fragments by means of polymerase chain reactions or their like with quantitative polymerase chain reaction version in the presence of two DNA and RNA standards one of which is introduced for analysis sensitivity control and in this way corresponds to duplicated pathogen genome fragment and is available in minimum quantities for being detected. The other one is introduced for controlling statistical significance of the result determined by the volume of sample participating in analysis and corresponds in this way to genetic marker describing this volume. The marker can be some predefined DNA or RNA fragment from host organism cells or special kind of bio-object introduced into the sample as one modeling the pathogen as well as genetic material fragment usable as carrier at the stage of sample fractionation before polymerase chain reaction being set, depending on material type to be analyzed. EFFECT: provided reliability of analysis results. 3 dwg

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RU 2 143 113 C1

Authors

Elov Andrej Aleksandrovich

Vinogradov Aleksandr Vladimirovich

Mednikov Boris Mikhajlovich

Makarov Nikolaj Vasil'Evich

Dates

1999-12-20Published

1996-08-01Filed