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2 208 645

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IPC

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Application

2002123004/13, 2002-08-28

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Start date

2002-08-28

(22)

Actual filing date

2002-08-28

(45)

Published

2003-07-20

(72)

Inventor

Cherkasov E.G.Kruglov A.N.Elov A.A.Fedorov N.A.Fedorov E.N.Sukhanov Ju.S.

(73)

Holder

Cherkasov Evgenij Gennad'EvichKruglov Aleksandr NikolaevichElov Andrej AleksandrovichFedorov Nikolaj AlekseevichFedorov Egor NikolaevichSukhanov Jurij Semenovich

METHOD OF DETECTION OF BACTERIAL BLOOD CONTAMINATION Russian patent published in 2003 - IPC

Abstract RU 2208645 C1

FIELD: medicine, veterinary science, food and pharmaceutical industry. SUBSTANCE: for assay carrying out DNA is isolated from blood sample and polymerase chain reaction (PCR) is carried out. Primers for nucleotide sequence that is general for all microorganisms are used in PCR and reaction is carried out in the presence of internal standard of competitive type. Signals from internal standard and for nucleotide sequence that is general for all microorganisms are detected. Bacterial blood contamination is determined in the case of simultaneous presence of signals from internal standard and from nucleotide sequence that is general for all microorganisms. The absence of bacterial contamination is determined in the case of signal from a single internal standard only. Invention can be used for direct gene testing bacterial contamination of donor blood and its components. EFFECT: enhanced precision of detection of contaminants. 5 dwg, 2 tbl, 4 ex

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RU 2 208 645 C1

Authors

Cherkasov E.G.

Kruglov A.N.

Elov A.A.

Fedorov N.A.

Fedorov E.N.

Sukhanov Ju.S.

Dates

2003-07-20—Published

2002-08-28—Filed