METHOD OF PREPARING ANTIGENIC PREPARATION HAEMOPHILUS INFLUENZAE TYPE B (HIB) Russian patent published in 2002 - IPC

FIELD: medicinal microbiology, vaccines. SUBSTANCE: for preparing an antigenic preparation type B (Hib) microorganisms are cultured in liquid nutrient media and exogenous polysaccharide is extracted from obtained biomass by ultrafiltration on molecular fiber filters. Cell concentrate is treated with of sodium chloride 2.5% solution with EDTA sodium salt and capsule polysaccharide Hib in its complex with protein is isolated from obtained extract by ultrafiltration followed by combination of capsule polysaccharide with exogenous polysaccharide Hib in ratio 1:1. Method ensures to prepare an antigenic preparation showing high antigenic activity that is useful for immunization and development of immunobiological preparation. Invention can be used for vaccine preparations preparing. EFFECT: improved method of preparing, high antigenic activity. 2 dwg, 3 tbl, 4 ex