FIELD: medicine.
SUBSTANCE: group of inventions relates to a method for producing a high titre of hepatitis E virus (HEV), comprising a) culturing a cell line in vitro, where the cell line is HepG2 (HB-8065 in the American Type Culture Collection (ATCC)) or HepG2/C3A (CRL-10741 in ATCC), in a medium containing polybrene in concentration of 1 mcg/ml to 5 mcg/ml, and b) infecting said cell line with HEV virus. Group of inventions also relates to a method of determining presence and / or level of HEV in a sample; culture medium for producing high HEV titre containing polybrene in range of 1 mcg/ml to 5 mcg/ml; method of examining HEV titration, comprising using a cell line, which is HepG2 (number HB-8065 in ATCC) or HepG2/C3A (number CRL-10741 in ATCC), and polybrene in range of 1 mcg/ml to 5 mcg/ml.
EFFECT: group of inventions provides high HEV virus titre.
16 cl, 5 ex, 5 dwg, 2 tbl
| Title | Year | Author | Number |
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| METHODS OF PURIFYING VIRUS PRODUCED IN VITRO, AND VIRUS ELIMINATION ANALYSIS | 2016 |
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| RECOMBINANT VESICULAR STOMATITIS VIRUS EXPRESSING CHIMERIC IL12-GMCSF PROTEIN | 2022 |
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| VIRAL PARTICLES CONTAINING ALPHAVIRUS VECTOR AND METHOD FOR PREPARING SAID VIRAL PARTICLE | 2004 |
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| CATTLE DIARRHEA VIRUS WITH MODIFIED E PROTEIN | 2009 |
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| RECOMBINANT VACCINE FOR PREVENTION OF VIRAL HEPATITIS E IN ANIMALS | 2012 |
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RU2501568C1 |
