FIELD: biotechnology. SUBSTANCE: invention relates to methods of production of protamine sulfate from milts of mature fishes. Method involves raw boiling out in water, milling, treatment with acetone, drying to granules formation followed by extraction of granules with 4% sulfuric acid solution, proteins precipitation from extract by addition of alcohol twofold volume, precipitate dissolving in hot water, treatment with barium hydroxide and addition of ammonia, treatment with kieselguhr, filtration, passing filtrate through cationite in H+-form, eluate concentration, precipitation of end product, its isolation and drying. EFFECT: improved processes of extraction and filtration in granules preparing. 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR MANUFACTURING PROTAMINE SULFATE (SALMINE) OUT OF MATURE SALMON SOFT ROE | 2003 |
|
RU2251427C1 |
| METHOD FOR PRODUCTION OF APYROGENIC IMMUNOMODULATING AGENT | 2006 |
|
RU2309759C1 |
| METHOD FOR PRODUCING IMMUNOTROPIC STERILE APYROGENIC TOLERANT PREPARATION BASED ON LOW-MOLECULAR NATIVE DEOXYRIBONUCLEIC ACID SODIUM SALT | 2003 |
|
RU2236853C1 |
| PROCESS FOR SODIUM PREPARING SALT POWDER OF DEOXYRIBONUCLEIC ACID FROM MILT | 1993 |
|
RU2041885C1 |
| METHOD OF PREPARING CHOLESTEROL-ESTERASE, TRIPSIN, DEOXYRIBONUCLEASE, AND RIBONUCLEASE FROM BOVINE PANCREAS | 2005 |
|
RU2311455C2 |
| PROCESS OF PRODUCTION OF SODIUM DNA SALT FROM ANIMAL RAW MATERIAL AND BIOREACTOR FOR ITS REALIZATION | 1993 |
|
RU2005724C1 |
| METHOD OF PREPARATION OF RHODIUM NITRATE SOLUTION | 2004 |
|
RU2265579C1 |
| PROCESS FOR CLEANING PLATING PLANT WASH WATER | 2004 |
|
RU2288185C2 |
| METHOD FOR PRODUCING DNA-CONTAINING PREPARATION FOR ORAL USE AND PREPARATION OBTAINED BY THIS METHOD | 2022 |
|
RU2779914C1 |
| METHOD OF NUCLEOPROTEIN COMPLEX PREPARING | 1995 |
|
RU2122856C1 |
