ANTIBODY RAISED AGAINST PROTEIN RELATIVE TO HUMAN PARATHYROID HORMONE, DNA-VECTOR, METHOD OF PREPARING AND ANTIBODY USING Russian patent published in 2003 - IPC

FIELD: biotechnology, medicine, pharmacy. SUBSTANCE: invention relates to immunoglobulins raised against PTPrH prepared by methods of genetic engineering. Invention relates also to the development of chimeric L- and H-chains of complete antibody based on thereof, humanized antibodies and polypeptides of L- and H-chains of region V, DNA encoding their and vector. Invention discloses methods of preparing chimeric and humanized antibodies and usage in pharmaceutical composition for prophylaxis and treatment of weight loss associated, in part, with malignant neoplasms. Antibody-base agent for inhibition of hypercalcimia and improvement of state in hypophosphatemia is developed. Invention ensures to obtain humanized antibodies exhibiting sufficient binding activity and neutralization of specific antigen (PTPrH) that is provided by means the development of effective methods of their preparing. EFFECT: improved method of antibody preparing, high binding activity. 51 cl, 31 dwg, 6 tbl