METHOD FOR OBTAINING PROTECTIVE EXTRACELLULAR BRUCELLA ANTIGEN CAPABLE TO PROVOKE CHRONIC FORMS OF BRUCELLOSIS Russian patent published in 2003 - IPC

Abstract RU 2199340 C1

FIELD: medicine, veterinary science. SUBSTANCE: protective antigen is characterized by sugars' content up to 60%, proteins up to 20%, nucleotides up to 5%, it has molecular weight below 30 kDa and maximum absorption within 250-265 nm wave lengths. Antigen is obtained during cultivation of Brucella in nutritive medium at 5.5- 7.0 pH. The former is isolated out of liquid fraction of culture liquid presubjected to either acidic or alkaline hydrolysis and purified due to ultrafiltration or liquid chromatography. The method provides to obtain antigen capable to provoke latent forms of brucellosis. EFFECT: higher efficiency. 3 cl, 12 ex, 2 tbl

Similar patents RU2199340C1

Title	Year	Author	Number
METHOD FOR OBTAINING OF ANTIGEN FOR DETERMINATION OF ANTIBRUCELLAR IMMUNITY	2016	Fedorov Andrej Ivanovich Iskandarov Marat Idrisovich Albertyan Mkrtich Pogosovich Iskandarova Salmikhanum Samurkhanovna Gulyukin Mikhail Ivanovich	RU2627897C1
METHOD FOR CARRYING OUT DIAGNOSTIC REACTION AT BRUCELLOSIS	2001	Ignatov P.E. Fedorov A.I. Mel'Nichenko L.P.	RU2203499C1
METHOD OF INFECTIOUS DISEASE TREATMENT	1993	Ignatov Petr Evgen'Evich	RU2092184C1
METHOD OF OBTAINING A BRUCELLAROTIC ANTIGEN FOR CELL TESTS IN VITRO	2019	Kurcheva Svetlana Aleksandrovna Ponomarenko Dmitrij Grigorevich Zharnikova Irina Viktorovna Kovalev Dmitrij Anatolevich Rakitina Ekaterina Lvovna Koshkidko Aleksandra Gennadevna Kostyuchenko Marina Vladimirovna Zhdanova Elena Vladimirovna Geogdzhayan Anna Samvelovna Logvinenko Olga Vasilevna	RU2708561C1
METHOD FOR PREVENTING AND TREATING BRUCELLOSIS	2002	Ignatov P.E. Fedorov A.I.	RU2230572C1
METHOD OF TREATMENT OF INFECTIOUS ENCEPHALITIS AND ENCEPHALOMYELITIS	1994	Ignatov Petr Evgen'Evich Fedorov Andrej Ivanovich	RU2098126C1
METHOD FOR PRODUCING AN ALLERGEN FOR DIAGNOSING BRUCELLOSIS IN FARM ANIMALS	2022	Najmanov Ali Khusinovich Iskandarov Marat Idrisovich Fedorov Andrej Ivanovich Iskandarova Salmikhanum Samurkhanovna Vangeli Elena Petrovna Tolstenko Nina Gavrilovna Myasoedov Yurij Mikhajlovich Gulyukin Aleksej Mikhajlovich	RU2792814C1
WAY OF RECEPTION OF BRUCELLOUS ANTIGEN FROM STRAIN BRUCELLA ABORTUS 19 FOR MANUFACTURING OF UNIFORM BRUCELLOUS ANTIGEN FOR AA (AGGLUTINATION ASSAY), CFR (COMPLEMENT FIXATION REACTION) AND LCFR (LONG-TERM COMPLEMENT FIXATION REACTION), BRUCELLOUS ANTIGEN FOR ROSE BENGAL ASSAY (RBA) AND BRUCELLOUS ANTIGEN FOR RING TEST (RT) WITH MILK, WAY OF MANUFACTURING OF BRUCELLOUS DIAGNOSTIC SERUM AND DIAGNOSTIC SETS	2008	Sobolev Viktor Vasil'Evich Mel'Nik Nikolaj Vasil'Evich Skljarov Oleg Dmitrievich Trojnin Anatolij Serafimovich Zenov Nikolaj Ivanovich Klimanov Arkadij Ivanovich Shumilov Konstantin Vasil'Evich Litenkova Irina Jur'Evna Rakhmanin Pavel Petrovich Krjukov Sergej Veniaminovich Trenev Vasilij Nikolaevich Solov'Ev Boris Vasil'Evich Balashov Vladimir Grigor'Evich	RU2361610C1
METHOD FOR DIFFERENTIAL DIAGNOSTICS OF BOVINE BRUCELLOSIS AND A METHOD OF PREPARING A PREPARATION FOR IMPLEMENTATION THEREOF	2005	Ivanov Arkadij Vasil'Evich Salmakov Konstantin Mikhajlovich Plotnikova Ehdie Minachetdinovna Nizamov Ramzi Nizamovich	RU2300107C2
METHOD FOR PREPARING CONCENTRATED BRUCELLA CELL CULTURE FROM Brucella abortus 19 STRAIN TO PREPARE BRUCELLOUS ANTIGENS, BRUCELLOUS ANTIGENS (THREE VERSIONS), METHOD OF MAKING BRUCELLOSIS DIAGNOSTIC SERUM AND TEST SYSTEM FOR DIAGNOSING BRUCELLOSIS IN ANIMALS (THREE VERSIONS)	2014	Trojnin Anatolij Serafimovich Elnikov Vasilij Viktorovich Kryukova Elena Nikolaevna Surnev Dmitrij Sergeevich Melnik Nikolaj Vasilevich Goldina Vera Fedorovna Litenkova Irina Yurevna Galkina Tatyana Sergeevna Klimanov Arkadij Ivanovich Sklyarov Oleg Dmitrievich Zenov Nikolaj Ivanovich	RU2593712C2

RU 2 199 340 C1

Authors

Ignatov P.E.

Fedorov A.I.

Dates

2003-02-27—Published

2001-09-12—Filed