METHOD FOR OBTAINING CLONING COWS Russian patent published in 2003 - IPC

FIELD: gene engineering. SUBSTANCE: The present innovation deals with methods for animal cloning due to obtaining the lines of cow's donor somatic cells, isolation of oocytes out of cow's ovary and their maturation in vitro. Then cumulus cells and surrounding oocytes should be removed to obtain enucleated recipient oocytes. Then one should transfer nucleus of donor cells into recipient oocyte followed by electrofusion and activation of electrofused cells that leads to obtaining an embryo. Then obtained embryos should be transferred into mother surrogate followed by obtaining a cloned calf. The present innovation is applied to manufacture pharmaceutical preparations and organs out of cloned cows and for carrying out research trials, as well. EFFECT: higher efficiency. 10 cl, 4 dwg, 9 ex, 12 tbl