FIELD: medicine.
SUBSTANCE: there are disclosed methods for producing human stem cells in parthenogenetic activation of human oocytes. The methods provide manipulation of O2 pressure involving manipulation of Ca2+ ion concentration at high O2 pressure and contact of oocytes with serine-threonine kinase inhibitor at low O2 pressure. It is followed by recovery of internal cell mass (ICM) from activated oocytes and culture of recovered ICM cells at high O2 pressure.
EFFECT: methods enable producing human stem cells which may be further used for research and medicine.
16 cl, 18 dwg, 19 tbl
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| IMPROVING ORGANS FOR TRANSPLANTATION | 2014 |
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| METHOD OF PRODUCING INDUCED PLURIPOTENT STEM CELLS FROM FIBROBLASTS OF PATIENTS WITH HUNTINGTON'S DISEASE | 2011 |
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RU2458983C1 |
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