METHOD FOR OBTAINING HUMAN CLONED EMBRYOS DUE TO APPLYING THE METHOD OF INTERSPECIFIC NUCLEAR TRANSPLANTATION Russian patent published in 2003 - IPC

FIELD: medicine, biology. SUBSTANCE: the present method deals with the following stages: obtaining the lines of human donor somatic cells; maturation of oocytes taken out of cow's ovary, in vitro; removal of oocyte- surrounding cumulus cells, dissection of the part of sparkling area of maturated oocytes by obtaining a fissure to press out the part of cytoplasm through this fissure including the first polar body at obtaining enucleated recipient oocytes; transportation of nucleus into recipient oocyte due to injection of donor cells into nucleus-free recipient oocytes followed by successive electrofusion and activation of electrofused cells to obtain embryos; postactivation and cultivation of embryos in vitro. Obtained cloned human embryos could be applied to obtain human embryonic stem cells to be widely used both in medicine and biology. EFFECT: higher efficiency. 12 cl, 4 dwg, 5 ex, 11 tbl