FIELD: biotechnology, microbiology, genetic engineering, biochemistry, amino acids. SUBSTANCE: method involves to preparing L-amino acids as such L-threonine, L-homoserine, L-methionine, L-arginine, L- proline, L-isoleucine or L-glutamic acid by culturing the microorganism Escherichia coli transformed with gene encoding enzyme pyruvate carboxylase being this strain E. coli shows ability to produce L-amino acids. Accumulated L-amino acids are extracted from nutrient medium. The strain of bacterium E. coli MG 442 or 44 is transformed with plasmid comprising the gene encoding the enzyme pyruvate carboxylase. By the first variant the strain produces L-threonine or L-glutamic acid and by the latter variant L-homoserine. Invention provides the high effectiveness of production of L-amino acids. EFFECT: improved preparing method, valuable properties of strain. 5 cl, 3 dwg, 1 tbl, 3 ex
