METHOD FOR PREPARING N-DEACYLATED CEPHALOSPORIN Russian patent published in 2003 - IPC

FIELD: antibiotics, microbiology, biotechnology. SUBSTANCE: invention relates to method for preparing N-deacylated cephalosporins by enzymatic preparing their 7-acylated analogues. Method involves fermentation of penicillin- or cephalosporin-producing microorganism able to producing β-lactam and expression of acyltransferase, expandase activity and, optionally, acetyltransferase and/or hydroxylase activity. Fermentation is carried out in the presence of precursor of side chain of the formula HOOC-X-(CH₂)_n- COOH (values X and n are given in the invention claim) or its salt, ester or amide. Derivative of acyl-7- cephalosporin is extracted from enzymatic broth, deacylated and 7-cephalosporin is extracted in crystalline form. EFFECT: increased yield of main product, reduced level of by-side products. 11 cl