METHOD FOR ENZYMATIC CEPHALOSPORIN PREPARING Russian patent published in 2003 - IPC

FIELD: biotechnology, microbiology, antibiotics. SUBSTANCE: invention relates to production of antibiotics. Method for preparing cephalosporin deacylated by 7-amino-group is carried out by fermentation using cephalosporin-producing microorganism in the presence of side chain precursor, extraction of N-substituted cephalosporin from enzymatic broth or liquid with organic solvent, reverse extraction of N-substituted cephalosporin with water, treatment of aqueous phase with dicarboxylate acylase and isolation of crystalline cephalosporin of the formula (I) given in the invention claim from conversion solution. Before extraction of N-substituted cephalosporin with organic solvent enzymatic broth or liquid is incubated in acid medium at elevated temperature. Except for, for improvement of washing the first organic extract is washed out with acidified water and/or by removal of side chain with precursor with organic solvent, and/or by treatment of cephalosporin aqueous solution obtained at one or some steps with carbon dioxide. Invention provides reduction of impurities of penicillin components. EFFECT: improved preparing method. 16 cl, 1 ex