METHOD FOR ISOLATION OF VITAMIN B Russian patent published in 2003 - IPC

FIELD: microbiological industry, vitamins. SUBSTANCE: invention relates to method for isolation of vitamin B₁₂ from bacterial mass. Method involves concentrating ammonia eluate using nanofiltration that is carried out in regimen of tangential (flow-type) filtration in value of transmembrane pressure from 0.02 to 0.05 MPa that provides the filtration rate of ammonia eluate in carrying out the concentration process from 15 to 20 l/m²•h. Nominal threshold for retention of nanofiltrating membrane is 400 Da that provides practically complete retention of vitamin B₁₂. Method provides the enhancement of yield and quality of preparation, to simplify technological process, to reduce raw and energy consumptions due to exclusions of the following stages: adsorption of vitamin of activated carbon, desorption from carbon with isopropyl alcohol an aqueous solution, distillation off of alcohol and evaporation of an aqueous concentrate. EFFECT: improved isolating method. 2 ex