FIELD: veterinary virology. SUBSTANCE: invention relates to preparing method for preparing antigens of avian adenoviruses. Method involves culturing adenoviruses and purification of antigens by sorption chromatography. Culturing adenoviruses is carried out in bird embryos. Sorption chromatography is carried out for a single stage that involves acidification of supernatant obtained after centrifugation to pH = 4.5-5.0 followed by its application on sorbent that is equilibrated preliminary with buffer solution with pH = 4.5-5.0. Then equilibrating buffer is passed through sorbent followed by elution of antigen from sorbent with buffer solution at pH = 9.0. Method allows preparing antigen of avian adenoviruses with yield 94-97% and purity 94-96%. Proposed method can be used for preparing antigen preparations of avian adenoviruses used for diagnosis and prophylaxis. EFFECT: improved preparing method, valuable properties of antigens. 2 cl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING SUBUNIT GENE ENGINEERING VACCINE AGAINST HEPATITIS B | 1992 |
|
RU2067767C1 |
| METHOD OF PRODUCING ADENOVIRAL ANTIGENS | 0 |
|
SU1364342A1 |
| PENTAVALENT SUBUNIT VACCINE AGAINST RESPIRATORY INFECTIONS AND METHOD OF ITS PREPARATION | 2022 |
|
RU2804948C2 |
| POLYANILINE AS SORBENTS FOR REMOVING VIRUSES, NON-VIRAL PROTEINS AND AS IMMUNOSORBENT BASE, METHOD OF REMOVING OR ATTACHING VIRUSES USING SAID SORBENTS, IMMUNOADSORPTION METHOD USING SAID SORBENTS, SORPTION METHOD USING SAID SORBENTS | 2007 |
|
RU2372951C2 |
| METHOD FOR PURIFYING MAMMALIAN AND HUMAN RECOMBINANT ADENOVIRUSES | 2011 |
|
RU2465327C1 |
| METHOD OF INSERTION OF HETERODUPLEX DNA INTO GENOME OF ADENOVIRUS CELO AND RECOMBINANT ADENOVIRAL VECTOR CELO/PUC19 | 1992 |
|
RU2031122C1 |
| EGG YIELD REDUCING SYNDROME-76 VIRUS STRAIN "BISS = 113" IN POULTRY FOR PREPARING VACCINE PREPARATIONS | 2002 |
|
RU2221865C1 |
| METHOD OF PRODUCING ANTIGEN OR ANTIGENS FOR PRODUCTION OF INFLUENZA VACCINE AND A VACCINE BASED ON IT | 2019 |
|
RU2710239C1 |
| METHOD OF PRODUCING TETRAVALENT SUBUNIT INFLUENZA VACCINE | 2019 |
|
RU2740751C1 |
| METHOD OF OBTAINING RECOMBINANT ANTIGEN MBP_RBD_6HIS OF SARS-CoV-2 VIRUS WITH C-TERMINAL AFFINITY TAG 6XHIS-TAG, INTENDED FOR USE AS COMPONENT OF REAGENT KIT FOR COVID-19 SERODIAGNOSTICS | 2023 |
|
RU2813324C1 |
