Patenton — panteon of patents

(19)

RU

(11)

2 218 408

(13)

C2

(51)

IPC

(21) (22)

Application

2001129918/13, 2001-11-05

(24)

Start date

2001-11-05

(22)

Actual filing date

2001-11-05

(45)

Published

2003-12-10

(72)

Inventor

Susloparov M.A.Susloparov I.M.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

RECOMBINANT PLASMID DNA PUL83HCMV PROVIDING EXPRESSION IN BACTERIAL CELLS ESCHERICHIA COLI OF RECOMBINANT PROTEIN COMPRISING IMMUNODOMINANT MOIETY OF PHOSPHOPROTEIN PP5 HCMV AND FRAGMENT OF β-GALACTOSIDASE Russian patent published in 2003 - IPC

Abstract RU 2218408 C2

FIELD: biotechnology, genetic engineering, molecular biology, biochemistry. SUBSTANCE: plasmid represents constructed in vitro recombinant plasmid DNA comprising fragment of gene UL83 of human cytomegalovirus (HCMV) encoding immunodominant moiety of phosphoprotein pp65 HCMV from 231 to 551 amino acid. Construction provides in cells of bacterium E. coli biosynthesis of recombinant protein comprising fragment pp65 HCMV and fragment of β-galactosidase E. coli with polyhistidine (6 His) motif for affinity purification that elicits antigenic properties of human cytomegalovirus. This purified recombinant protein can be used as HCMV-antigen for serological testing HCMV in clinical practice. Use of this antigen for detection of virus-specific immunoglobulins M (IgM - HCMV) shows 98- 99% of sensitivity and specificity in immunoenzyme analysis. EFFECT: valuable biological and medicinal properties of antigen. 6 dwg, 3 ex

Similar patents RU2218408C2

Title Year Author Number
RECOMBINANT PLASMID DNA PUL 32 HCMV DETERMINING EXPRESSION OF HUMAN CYTOMEGALOVIRUS GENE UL 32 FRAGMENT ENCODING HYDROPHILIC SITE OF BASIC MATRIX PROTEIN PP 150 IN ESCHERICHIA COLI BACTERIUM CELLS 1998
  • Susloparov M.A.
 RU2151801C1
RECOMBINANT PLASMID DNA PU11HHV6 PROVIDING EXPRESSION OF GENE U11 FRAGMENT OF HUMAN HERPES VIRUS OF 6 TYPE ENCODING IMMUNODOMINANT MOIETY OF PROTEIN TEGUMENT P100 IN CELLS OF BACTERIUM ESCHERICHIA COLI 2000
  • Susloparov M.A.
  • Bakhtina M.M.
  • Susloparov I.M.
  • Makhova N.M.
  • Pljasunov I.V.
  • Lokteva L.A.
 RU2189393C2
RECOMBINANT PLASMID DNA PU27HHV6 PROVIDING EXPRESSION OF GENE U27 OF HUMAN HERPES VIRUS OF 6-TH TYPE ENCODING PROTEIN P41 IN BACTERIAL CELLS OF ESCHERICHIA COLI 2001
  • Susloparov M.A.
  • Susloparov I.M.
  • Pljasunov I.V.
  • Makhova N.M.
  • Bakhtina M.M.
 RU2201450C2
RECOMBINANT PLASMID DNA PUL 44 HCMV DETERMINING EXPRESSION OF HUMAN CYTOMEGALOVIRUS GENE UL 44 FRAGMENT ENCODING IMMUNODOMINANT SITE OF DNA-BINDING PROTEIN P 52 (ICP 36) IN ESCHERICHIA COLI BACTERIUM CELLS 1998
  • Susloparov M.A.
  • Susloparov I.M.
  • Pljasunov I.V.
 RU2151800C1
VIRAL GENOME FRAGMENT OF CHICKEN K STRAIN VARIOLA WHICH CONTAINS THYMIDINE KINASE GENE, pTK1FPV AND pTK2FPV RECOMBINANT PLASMID DNAs WHICH CONTAIN THIS SEQUENCE, AND pINTFPV1 RECOMBINANT PLASMID DNA WHICH IS ABLE TO INTEGRATE INTO VIRAL GENOME OF CHICKEN VARIOLA 1999
  • Susloparov M.A.
  • Pljasunov I.V.
  • Bakhtina M.M.
  • Susloparov I.M.
  • Makhova N.M.
  • Lokteva L.A.
 RU2180352C2
METHOD OF PRODUCING S-(+)-2,2-DIMETHYLCYCLOPROPANE CARBOXAMIDE, DNA FRAGMENT ENCODING R-(-)-2,2-DIMETHYLCYCLOPROPANE- -CARBOXAMIDE HYDROLASE OF COMAMONAS, RECOMBINANT PLASMID DNA (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI (VARIANTS) 1992
  • Tomas Tsimmermann
  • Karen Robins
  • Olven M.Behrch
  • Ehlizabet Belen
 RU2126450C1
RECOMBINANT PLASMID DNA PUR 290 SΔE ENCODING HYBRID POLYPEPTIDE β-GALACTOSIDASE-NS 1 - A PROTEIN OF MITE ENCEPHALITIS VIRUS, AND A METHOD OF ITS CONSTRUCTION 1989
  • Pugachev K.V.
  • Pletnev A.G.
  • Jamshchikov V.F.
  • Gorn V.V.
 RU1679798C
STRAIN OF HYBRID ANIMAL CELLS MUS MUSCULUS L5F10-PRODUCER OF MONOCLONAL ANTIBODIES USED FOR DETECTING PP65 PROTEIN OF HUMAN CYTOMEGALOVIRUS 2008
  • Kazachinskaja Elena Ivanovna
  • Susloparov Mikhail Aleksandrovich
  • Loktev Valerij Borisovich
 RU2393219C1
RECOMBINANT PLASMID DNA PUR 291-HAV 22 ENCODING SYNTHESIS OF HYBRID POLYPEPTIDE OF HEPATITIS VIRUS A ANTIGENIC DETERMINANTS WITH β-GALACTOSIDASE 1986
  • Ovchinnikov Ju.A.
  • Sverdlov E.D.
  • Tsarev S.A.
  • Rokhlina T.O.
  • Azhikina T.L.
  • Arsenjan S.G.
  • Kusov Ju.Ju.
  • Grabko V.I.
  • Nastashenko T.A.
  • Balajan M.S.
  • Drozdov S.G.
  • Chizhikov V.E.
  • Petrov N.A.
  • Vasilenko S.K.
  • Prikhod'Ko G.G.
 SU1380209A1
METHOD OF PRODUCING OXYTOCIN AND RECOMBINANT PLASMID DNA PTEILOX4 AND STRAIN OF ESCHERICHIA COLI BL21 (DE3)/PTEILOX4 FOR ITS REALIZATION 1999
  • Gurevich A.I.
  • Esipov R.S.
  • Miroshnikov A.I.
  • Kajushin A.L.
  • Shvets S.V.
  • Kostromina T.I.
 RU2161200C1

RU 2 218 408 C2

Authors

Susloparov M.A.

Susloparov I.M.

Dates

2003-12-10—Published

2001-11-05—Filed

download Download PDF read Similar patents