Patenton — panteon of patents

(19)

RU

(11)

2 201 450

(13)

C2

(51)

IPC

(21) (22)

Application

2001117662/13, 2001-06-25

(24)

Start date

2001-06-25

(22)

Actual filing date

2001-06-25

(45)

Published

2003-03-27

(72)

Inventor

Susloparov M.A.Susloparov I.M.Pljasunov I.V.Makhova N.M.Bakhtina M.M.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

RECOMBINANT PLASMID DNA PU27HHV6 PROVIDING EXPRESSION OF GENE U27 OF HUMAN HERPES VIRUS OF 6-TH TYPE ENCODING PROTEIN P41 IN BACTERIAL CELLS OF ESCHERICHIA COLI Russian patent published in 2003 - IPC

Abstract RU 2201450 C2

FIELD: biotechnology, genetic engineering. SUBSTANCE: invention proposes recombinant plasmid DNA pU27HHV6 that is obtained on the base of plasmid DNA pUR290. Plasmid DNA pU27HHV6 comprises gene U27 fragment of human herpes virus of 6-th type encoding protein p41. Construction provides synthesis of the end antigen at the level 40-50% with the yield of purified product after affinity chromatography up to 98-99% of total cellular proteins. The prepared recombinant protein exhibits high level of sensitivity and specificity in IFA used for detection of human blood immunoglobulins specific to HHV-6. EFFECT: valuable biological properties of protein. 6 dwg, 3 ex

Similar patents RU2201450C2

Title Year Author Number
RECOMBINANT PLASMID DNA PU11HHV6 PROVIDING EXPRESSION OF GENE U11 FRAGMENT OF HUMAN HERPES VIRUS OF 6 TYPE ENCODING IMMUNODOMINANT MOIETY OF PROTEIN TEGUMENT P100 IN CELLS OF BACTERIUM ESCHERICHIA COLI 2000
  • Susloparov M.A.
  • Bakhtina M.M.
  • Susloparov I.M.
  • Makhova N.M.
  • Pljasunov I.V.
  • Lokteva L.A.
 RU2189393C2
RECOMBINANT PLASMID DNA PUL 32 HCMV DETERMINING EXPRESSION OF HUMAN CYTOMEGALOVIRUS GENE UL 32 FRAGMENT ENCODING HYDROPHILIC SITE OF BASIC MATRIX PROTEIN PP 150 IN ESCHERICHIA COLI BACTERIUM CELLS 1998
  • Susloparov M.A.
 RU2151801C1
RECOMBINANT PLASMID DNA PUL 44 HCMV DETERMINING EXPRESSION OF HUMAN CYTOMEGALOVIRUS GENE UL 44 FRAGMENT ENCODING IMMUNODOMINANT SITE OF DNA-BINDING PROTEIN P 52 (ICP 36) IN ESCHERICHIA COLI BACTERIUM CELLS 1998
  • Susloparov M.A.
  • Susloparov I.M.
  • Pljasunov I.V.
 RU2151800C1
RECOMBINANT PLASMID DNA PUL83HCMV PROVIDING EXPRESSION IN BACTERIAL CELLS ESCHERICHIA COLI OF RECOMBINANT PROTEIN COMPRISING IMMUNODOMINANT MOIETY OF PHOSPHOPROTEIN PP5 HCMV AND FRAGMENT OF β-GALACTOSIDASE 2001
  • Susloparov M.A.
  • Susloparov I.M.
 RU2218408C2
VIRAL GENOME FRAGMENT OF CHICKEN K STRAIN VARIOLA WHICH CONTAINS THYMIDINE KINASE GENE, pTK1FPV AND pTK2FPV RECOMBINANT PLASMID DNAs WHICH CONTAIN THIS SEQUENCE, AND pINTFPV1 RECOMBINANT PLASMID DNA WHICH IS ABLE TO INTEGRATE INTO VIRAL GENOME OF CHICKEN VARIOLA 1999
  • Susloparov M.A.
  • Pljasunov I.V.
  • Bakhtina M.M.
  • Susloparov I.M.
  • Makhova N.M.
  • Lokteva L.A.
 RU2180352C2
PAIR OF SYNTHETIC OLIGONULCLEOTIDE-PRIMERS USED FOR DETECTION OF DNA OF HUMAN HERPES SIMPLEX OF TYPE 6 2001
  • Susloparov M.A.
  • Susloparov I.M.
  • Pljasunov I.V.
  • Makhova N.M.
  • Bakhtina M.M.
 RU2193067C1
RECOMBINANT PLASMID DNA P7SHSHIS FOR PRODUCTION OF DIMERIC SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF ESCHERICHIA COLI AS PRODUCER OF DIMERIC FORM OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS 2000
  • Nikolenko G.N.
  • Tikunova N.V.
  • Protopova E.V.
  • Loktev V.B.
  • Karpenko L.I.
  • Konovalova S.N.
  • Il'Ichev A.A.
 RU2190017C2
RECOMBINANT PLASMID DNA PKHBC-33 CONTAINING GENE OF HEPATITIS B VIRUS CORE ANTIGEN INVOLVING SEQUENCE OF THE MAIN NEUTRALIZING EPITOPE OF HEPATITIS B VIRUS SURFACE ANTIGEN (137-147) AND STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF HEPATITIS B VIRUS CORE ANTIGEN WITH THE MAIN NEUTRALIZING EPITOPE OF HEPATITIS B VIRUS SURFACE ANTIGEN EXHIBITED ON ITS SURFACE 1995
  • Karpenko L.I.
  • Chikaev N.A.
  • Melamed N.V.
  • Il'Ichev A.A.
  • Bajborodin S.I.
 RU2112039C1
RECOMBINANT PLASMID DNA PUHBC ENCODING GENE OF HEPATITIS VIRUS B CORE ANTIGEN PROTEIN AND ENSURING TO EXHIBIT FOREIGN EPITOPES ON THE CORE SURFACE 1995
  • Karpenko L.I.
  • Chikaev N.A.
  • Il'Ichev A.A.
 RU2121504C1
RECOMBINANT PLASMID DNA PQ-F35 ENCODING FUSED POLYPEPTIDE F35 SHOWING ANTIGENIC AND IMMUNOGENIC PROPERTIES OF MARBURG VIRUS PROTEIN VP 35, METHOD OF ITS PREPARING AND STRAIN OF BACTERIUM ESCHERICHIA COLI - SUPERPRODUCER OF RECOMBINANT POLYPEPTIDE F35 1998
  • Sorokin A.V.
  • Razumov I.A.
  • Kazachinskaja E.I.
  • Kachko A.V.
  • Ivanova A.V.
  • Mishin V.P.
  • Bukreev A.A.
  • Belanov E.F.
  • Loktev V.B.
  • Netesov S.V.
 RU2144565C1

RU 2 201 450 C2

Authors

Susloparov M.A.

Susloparov I.M.

Pljasunov I.V.

Makhova N.M.

Bakhtina M.M.

Dates

2003-03-27—Published

2001-06-25—Filed

download Download PDF read Similar patents