FIELD: biochemistry, proteins, hormones. SUBSTANCE: invention relates to method for purification of insulin by chromatography method. Mobile phase comprises at least one water-mixable organic solvent and at least one buffer substance with pH value from 7 to 11. Stationary phase represents pressure-stable organic polymeric chromatography material with average particles size from 10 to 50 mcm. Method provides the enhancement degree of insulin separation and reduced number of stages in purification process. EFFECT: improved purification method. 17 cl, 5 tbl, 4 ex
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