FIELD: medicine.
SUBSTANCE: invention relates to a method of purifying protein containing insulin and one polysialic acid residue from reaction by-products and unreacted starting material, using reverse-phase chromatography. Chromatographic material PLRP-S is used, which is balanced with a solution with ethanol content of 2–8 %, and elution with a step gradient.
EFFECT: invention ensures high protein purity per one stage of purification.
1 cl, 6 dwg, 3 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PURIFYING PEGYLATED ERYTHROPOIETIN | 2011 |
|
RU2566267C2 |
| N-TAILED POLYSIALYLATION | 2007 |
|
RU2432175C2 |
| HUMAN OXYNTOMODULIN, ITS APPLICATION, MEDICATION BASED THEREON AND METHOD OF APPLYING MEDICATION FOR TREATMENT AND PREVENTION OF HYPERGLYCEMIA | 2009 |
|
RU2524204C2 |
| INSULIN PURIFICATION | 2012 |
|
RU2603752C2 |
| BLOOD COAGULATION PROTEIN CONJUGATES | 2010 |
|
RU2744370C2 |
| CONJUGATES OF BLOOD COAGULATION PROTEINS | 2010 |
|
RU2595442C2 |
| ALDEHYDE DERIVATIVES OF SIALIC ACID, METHODS OF THEIR OBTAINMENT, CONJUGATES OF ALDEHYDE DERIVATIVES OF SIALIC ACID, AND PHARMACEUTICAL COMPOSITION BASED ON THEM | 2004 |
|
RU2333223C2 |
| RECOMBINANT PLASMID DNA pMSIN4, CODING HYBRIDE POLYPEPTIDE - HUMAN INSULIN PRECURSOR, BL21(DE3)VpMSIN4-PRODUCER STRAIN OF RECOMBINANT HUMAN INSULIN, METHOD FOR PRODUCING RECOMBINANT HUMAN INSULIN | 2011 |
|
RU2447149C1 |
| METHOD FOR PREPARING HIGH-PURITY CRYSTALLINE INSULIN OF ANY ORIGIN | 2011 |
|
RU2453331C1 |
| METHOD FOR PRODUCTION OF CHEMICALLY POLYSIALYLATED RECOMBINANT HUMAN BUTYRYLCHOLIN ESTERASE | 2012 |
|
RU2645458C2 |
