METHOD OF POLYSIALYLATED HUMAN INSULIN PURIFICATION Russian patent published in 2020 - IPC C07K1/20 C07K14/62 A61K38/28 

Abstract RU 2736358 C2

FIELD: medicine.

SUBSTANCE: invention relates to a method of purifying protein containing insulin and one polysialic acid residue from reaction by-products and unreacted starting material, using reverse-phase chromatography. Chromatographic material PLRP-S is used, which is balanced with a solution with ethanol content of 2–8 %, and elution with a step gradient.

EFFECT: invention ensures high protein purity per one stage of purification.

1 cl, 6 dwg, 3 tbl, 3 ex

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RU 2 736 358 C2

Authors

Avtushenko Sergej Sergeevich

Surkov Kirill Gennadievich

Petrov Andrej Valerevich

Yan Sergej Fedorovich

Lazarev Vyacheslav Sergeevich

Gavrilova Evgeniya Nikolaevna

Dates

2020-11-16Published

2015-12-09Filed