METHOD FOR OBTAINING CERULOPLASMIN Russian patent published in 2004 - IPC

FIELD: medicine. SUBSTANCE: the present innovation deals with technology to obtain medicinal preparations out of human blood plasma. Residue of VI-1 alcoholic fraction of blood plasma should be homogenized by special technique in sodium chloride solution followed by thermal denaturation of protein admixtures, sorption of obtained solution upon DEAE-cellulose, elution with sodium chloride solution at concentration being 0.1 M, not higher, precipitation of admixtures with ethanol and chloroform mixture, their separation with centrifuging, precipitation of target product with ethanol, dissolving the residue, sterilizing filtration, pasteurization and lyophilic drying of ceruloplasmin solution. At the stage of dissolving the residue of target product one should add mannitol into solution at the quantity of 0.05-0.15 mg/ml protein that enables to obtain preparation of high enzymatic activity being 12 U/mg, not less. EFFECT: higher efficiency. 2 ex