FIELD: molecular biology, virology, medicinal biotechnology.
SUBSTANCE: invention is designated for carrying out the express-diagnosis of orthopox-viral infections using multiplex PCR (polymerase chain reaction)-analysis. Primers pairs with specificity for each species of orthopox viruses are selected. Administration of additional primer pair to high-conservative region of these viruses as a positive control provides enhancing the sensitivity of method and to reduce probability for appearance of pseudonegative results. This approach provides improving the procedure of PCR-analysis for conventional using in diagnosis of orthopox-viral infections due to application of a single multiplex PCR-analysis. Multiplex PCR-analysis provides detecting viral material in a single reaction mixture without using additional methods of analysis that results to reducing time for diagnosis and percent of technological errors.
EFFECT: improved method for analysis, valuable biological properties of set.
2 tbl, 10 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBES FOR SPECIES-SPECIFIC INSTANT IDENTIFICATION OF ORTHOPOXVIRUS BY REAL-TIME MULTIPLEX PCR | 2010 |
|
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| RECOMBINANT PLASMID pFastBAC-B17R DNA CONTAINING COWPOX VIRUS GENOME FRAGMENT CODING ALPHA/BETA-INTERFERON BINDING PROTEIN AND BvB17RG BACULOVIRUS STRAIN PRODUCING SOLUBLE ALPHA/BETA-INTERFERON BINDING PROTEIN OF COWPOX VIRUS | 2009 |
|
RU2405824C1 |
| KIT OF PRIMER OLIGONUCLEOTIDES, BIOARRAY AND METHOD FOR SPECIFIC IDENTIFICATION OF PATHOGENIC FOR HUMAN ORTHOPOX- AND HERPESVIRUSES | 2006 |
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| STRAIN "VNIIZZH 2003" OF GOAT-POX VIRUS VARIOLA VIRUS CAPRINUM TO PRODUCE BIOPREPARATIONS FOR DIAGNOSTICS AND SPECIFIC PROPHYLACTICS OF GOAT-POX | 2008 |
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| VIRUS-VACCINE ASSOCIATED AGAINST SHEEP POX AND GOAT POX CULTURE DRY | 2009 |
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| SET OF OLIGONUCLEOTIDE PRIMERS FOR DETECTING DNA OF ORTHOPOXVIRUSES BY ISOTHERMAL AMPLIFICATION IN REAL TIME | 2024 |
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RU2823776C1 |
| RECOMBINANT STAIN OF VACΔ6 VACCINIA VIRUS WITH BROKEN GENES OF VIRALENCE C3L, N1L, J2R, A35R, A56R, B8R FOR THE PRODUCTION OF LIVE CULTURAL ATTENUATED VACCINE AGAINST NATURAL SMALLPOX AND OTHER ORTHOPOXVIRAL HUMAN INFECTIONS | 2016 |
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RU2621868C1 |
| TISSUE-CULTURE DRY GOAT-POX VIRUS-VACCINE | 2008 |
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| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBES FOR SPECIES-SPECIFIC INSTANT IDENTIFICATION OF MARBURG VIRUS BY POLYMERASE CHAIN REACTION | 2011 |
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| SET OF GENUS- AND SPECIES-SPECIFIC OLIGONUCLEOTIDE PRIMERS FOR DETECTION AND SPECIES IDENTIFICATION OF YERSINIA BY MULTIPLEX POLYMERASE CHAIN REACTION METHOD | 2006 |
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RU2354700C2 |
