METHOD FOR PREPARING GENETICALLY MODIFIED POTATO PLANTS OF STRAIN "ELIZAVETA" USING AGROBACTERIUM TUMEFACIENS Russian patent published in 2004 - IPC

FIELD: agriculture, biotechnology and selection of plants.

SUBSTANCE: explants of potato plant of the strain "ELIZAVETA" are co-cultured with the transformed strain A. tumefaciens and placed then in nutrient medium for inducing callus-formation followed by regeneration of fertile transgenic plants from them. Co-cultivation is carried out by contact of explants with a filter wetted with bacterial suspension for 48 ± 4 h. For inducing callus-formation the nutrient medium containing zeatin taken in the amount from 0.6 to 1.0 ± 0.5 mg/l; benzylaminopurine taken in the amount from 0.6 to 1.0 ± 0.5 mg/l and naththyl acetic acid or idolyl acetic acid taken in the amount from 0.01 to 0.05 mg/l is used. On 8-th day explants are placed in the regenerating medium containing zeatin taken in the amount from 0.5 to 3.5 mg/l and gibberellic acid (GA₃) taken in the amount from 0.01 to 0.05 mg/l.

EFFECT: improved preparing method.

5 cl, 1 dwg, 5 tbl, 1 ex