FIELD: agriculture, plant selection.
SUBSTANCE: explants of potato plants of the strain "Golubizna" are co-cultured with the transformed strain A. tumefaciens and placed then on nutrient medium for inducing callus-formation followed by regeneration of fertile transgenic plants from them. Co-culturing is carried out by contact of explants with a filter placed on surface of agarized medium and with bacterial suspension applied on medium for 48 ± 4 h. For induction of callus-formation the nutrient medium containing benzylaminopurine taken in the amount 2.5-5.5 mg/l and naththyl acetic acid taken in the amount 0.01-0.05 mg/l is used. The regeneration is begun on 8-th day in medium containing benzylaminopurine taken in the amount 2.5-5.5 mg/l and gibberellic acid (GA3) taken in the amount 0.01-0.05 mg/l. Method provides increase yield of transformed plants.
EFFECT: improved preparing method.
5 cl, 2 dwg, 4 tbl, 1 ex
