METHOD FOR PREPARING GENETICALLY MODIFIED POTATO PLANTS OF STRAIN "GOLUBIZNA" USING AGROBACTERIUM TUMEFACIENS Russian patent published in 2004 - IPC

Abstract RU 2231551 C2

FIELD: agriculture, plant selection.

SUBSTANCE: explants of potato plants of the strain "Golubizna" are co-cultured with the transformed strain A. tumefaciens and placed then on nutrient medium for inducing callus-formation followed by regeneration of fertile transgenic plants from them. Co-culturing is carried out by contact of explants with a filter placed on surface of agarized medium and with bacterial suspension applied on medium for 48 ± 4 h. For induction of callus-formation the nutrient medium containing benzylaminopurine taken in the amount 2.5-5.5 mg/l and naththyl acetic acid taken in the amount 0.01-0.05 mg/l is used. The regeneration is begun on 8-th day in medium containing benzylaminopurine taken in the amount 2.5-5.5 mg/l and gibberellic acid (GA3) taken in the amount 0.01-0.05 mg/l. Method provides increase yield of transformed plants.

EFFECT: improved preparing method.

5 cl, 2 dwg, 4 tbl, 1 ex

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Authors

Starodubtseva A.M.

Belousova M.B.

Konov A.L.

Skrjabin K.G.

Dates

2004-06-27Published

2002-04-24Filed