METHOD FOR PREPARING GENETICALLY MODIFIED POTATO PLANT OF STRAIN "NEVSKIY" USING AGROBACTERIUM TUMEFACIENS Russian patent published in 2004 - IPC

FIELD: agriculture, plant selection.

SUBSTANCE: explants of potato plant of the strain "Nevskiy" are co-cultured with the transformed strain A. tumefaciens and placed then in the nutrient medium for inducing callus-formation followed by regeneration of fertile transgenic plants from them. Co-culturing is carried out by contact of explants through a paper filter with surface of agarized medium with the applied bacterial strain for 48 ± 4 h. For induction of callus-formation explants are placed in nutrient medium containing zeatin taken in the amount 0.5-3.5 mg/l and naththyl acetic acid taken in the amount 0.01-0.05 mg/l. On the 8-th day explants are placed in the regenerating medium containing zeatin taken in the amount 0.5-3.5 mg/l and gibberellic acid (GA₃₎ taken in the amount 0.01-0.05 mg/l. Invention provides increasing yield of transformed plants.

EFFECT: improved preparing method.

5 cl, 2 dwg, 5 tbl, 1 ex