FIELD: medicinal immunology.
SUBSTANCE: invention relates to methods for assay of functional activity of complement factors in human serum blood and can be used in diagnosis of some diseases and in biological preparations. Method involves sorption of activator of the complement alternative pathway into microplate wells, then solution containing reagent RD and analyzed sample containing human complement factor D with the unknown activity are added. Incubation is carried out in the presence of Mg2+ ions and in the absence of Ca2+ ions. Then conjugate of enzyme with antibodies raised against human component C3 and substrate for this enzyme are added. The calculation of factor D activity is carried out by the amount of product of enzymatic reaction. Invention provides the development of method allowing the determination of the functional activity of factor D in alternative pathway of the human complement system.
EFFECT: improved assay method.
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