FIELD: measuring equipment.
SUBSTANCE: presented group of inventions refers to medicine. There are presented method and kit for the immune-enzyme assay of functional activity of human complement component C3. The method implies thrombin sorption in microplate wells, introduction of a test sample containing the human complement component C3 with unknown activity, incubation in the presence of ethylene diamine tetraacetate (EDTA), pouring out of the well contents, introduction of an enzyme conjugate with human complement component C3 antibodies and a substrate of the above enzyme. The component C3 activity is evaluated by the amount of the prepared product of enzymatic reaction. The kit comprises the flat-bottomed microplate with sorbed thrombin, conjugated enzyme and human complement component C3 antibodies, substrate buffer and donor blood serum with known activity C3 as a reference.
EFFECT: presented group of inventions enables evaluating the functional activity of human complement component C3 without the need of activation of the whole complement system, and possesses a good result reproducibility.
2 cl, 1 dwg, 2 ex
