FIELD: medicine.
SUBSTANCE: invention is related to the field of medicine and method and set for immune-enzyme detection of functional activity of factor D of human complement. Substance of invention includes introduction of lysozyme into micropanel holes as activator of alternative path of complement, then introduction of RD reagent solution and analysed sample, further incubation is carried out in presence of Mg2+ ions and absence of Ca2+ ions, and then conjugate of ferment is introduced with antibodies against human component C3, as well as substrate of this ferment, and activity of factor D is calculated by number of generated product of fermentative reaction. Set for detection of functional activity of factor D of human complement contains flat-bottomed micropanel with absorbed lysozyme, reagent RD (serum of human blood, devoid of factor D activity), conjugate of peroxidase with antibodies to component C3 of human complement, substrate buffer and standard with available activity of factor D.
EFFECT: improved sensitivity of method.
2 cl, 2 ex, 1 dwg
