FIELD: medicine.
SUBSTANCE: invention relates to methods used for cryopreserving human hemopoietic cells. Cryoprotecting agent dimethylsulfoxide is added to cell suspension to its final concentration 0.4-1.45 mole/l and three-step freezing the suspension is carried out followed by its thawing. On the first step freezing is carried out at the rate in the range 0.7-1.2 C/min to temperature (-8.5)-(-10.5) C using siding at temperature (-3.5)-(-4) C; on the second step - at the rate 1.0-4.5 C/min to (-30)-(-40) C and after the second step temperature is stopped at (-30)-(-40) C for 3-5 min; on the third step - at the rate 10-12 C/min to (-130)-(-196) C. Method provides elongation of storing period and to increase the amount of viable cells.
EFFECT: improved cryopreserving method.
2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF CRYOPRESERVATION OF HEMATOPOIETIC STEM CELLS OF UMBILICAL BLOOD | 2009 |
|
RU2416197C1 |
| METHOD FOR HUMAN NUCLEATED BLOOD CELLS CRYOPRESERVATION AT TEMPERATURE OF MINUS 80°C | 2017 |
|
RU2639892C1 |
| METHOD FOR CRYOGENIC PRESERVATION OF HAEMATOPOIETIC UMBILICAL CORD STEM CELLS | 2012 |
|
RU2554405C2 |
| METHOD FOR ADRIFT FREEZING OF PERIPHERAL BLOOD PRECURSOR CELLS AT A TEMPERATURE OF MINUS 80 °C WITH A SOLUTION OF HEMODIOLITE | 2019 |
|
RU2716574C1 |
| METHOD FOR UNSTRUCTURED FREEZING PERIPHERAL BLOOD PRECURSOR CELLS AT MINUS 80 °C WITH A HEMICOLITE SOLUTION | 2019 |
|
RU2711152C1 |
| METHOD OF PRESERVING LEUKOCYTES | 0 |
|
SU825081A1 |
| METHOD FOR CRYOPRESERVATION OF HAEMOPOIETIC STEM CELLS | 2016 |
|
RU2624214C1 |
| METHOD FOR PRESERVING VIABILITY OF HEMATOPOIETIC STEM CELLS OF UMBILICAL BLOOD AFTER WASHING FROM CRYOPROTECTANT | 2023 |
|
RU2821586C1 |
| METHOD FOR CRYOPRESERVATION OF HAEMOPOIETIC UMBILICAL STEM CELLS | 2013 |
|
RU2547426C1 |
| METHOD FOR UNREGULATED FREEZING OF PERIPHERAL BLOOD PRECURSOR CELLS AT TEMPERATURE OF MINUS 80 °C WITH GEMGEL SOLUTION | 2018 |
|
RU2720771C1 |
