METHOD FOR ADRIFT FREEZING OF PERIPHERAL BLOOD PRECURSOR CELLS AT A TEMPERATURE OF MINUS 80 °C WITH A SOLUTION OF HEMODIOLITE Russian patent published in 2020 - IPC A01N1/02 C12N5/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to preservation of cell culture. For freezing peripheral blood precursor cells (PBPC), freshly harvested nuclear cell concentrate (NCC) is recovered, mixing of NCC with cryopreserved Hecomoldiolite (HD) in polymer cryopacket (PC) in equal proportions, its sealing, marking, PC placing in metal holder-holder, PBPC in presence of HD from plus 4 °C to minus 80 °C according to three-stage program in electric refrigerators with set temperature of isothermic cold adaptation of cells (T_A), at the first stage of freezing, the holder with PBPC is placed for 10 minutes into an electric freezer with T_A plus 4 ± 1 °C, at the second stage the holder is transferred for 10 minutes into an electric freezer with T_A minus 7 ± 1 °C, at the third stage the holder is transferred into an electric freezer with T_A minus 28 ± 1 °C, held in these conditions for 25 minutes, then transferred into a storage-freezer with temperature minus 80 ± 2 °C for complete freezing of cell suspension and prolonged preservation.

EFFECT: invention allows improving safety of nuclear cells.

1 cl, 1 tbl, 1 ex