FIELD: molecular biology, biochemistry, diagnostic systems useful in detection of certain genes as well as known or unknown gene mutation in biological samples.
SUBSTANCE: invention relates to method for characterization of nucleic acid molecule sequence by detection of variation presence (or absence) in this sequence and characterization of detected variations. Claimed method includes enzymatic copying of sequence being interested in presence of at least one kind of dNTF with modified base which represents DNA-glycosilase substrate; treatment of obtained copy with DNA-glycosilase and further with agent cleaving the sequence to form fragments having OH-group on 3'-end4 incubation of said fragments in presence of matrix nucleic acid and enzyme being capable to provide tailing thereof; and extension product analysis.
EFFECT: testing and diagnosis methods with improved effectiveness.
20 cl, 13 dwg, 4 ex
