METHOD TO PRODUCE LYPOPOLYSACCHARIDE OF PLAGUE AGENT Russian patent published in 2013 - IPC C12P19/04 A61K39/02 A61K39/39 

FIELD: biotechnologies.

SUBSTANCE: invention may be used for production of antigens, diagnostic and preventive preparations. The method includes preliminary water and salt treatment of bacterial cells with their subsequent lysis by a buffer solution, containing 0.1 M Teis-HCI pH 8.0, 10 mM EDTA, 1% Triton X-100. Destruction with ultrasound is carried out. The untreated extract of LPS is treated with a ferment complex of proteovibrin until the final concentration of 160 mcg/ml and incubated at 37°C, pH 7.8-8.0 for 18 h. Treatment from nucleic acids is carried out by acidification of a sample with an ice acetic acid to pH 3.2-3.4 and their deposition at 5000 g for 30 min.

EFFECT: invention makes it possible to improve quality of LPS preparations, to simplify and cheapen methodology, to reduce time of LPS extraction.

2 tbl, 2 dwg