PROTEIN BINDING SPECIFICALLY WITH OSTEOCLASTOGENESIS-INHIBITING FACTOR (OCIF) (VARIANTS), DNA ENCODING ITS (VARIANTS), DNA AS PROBE (VARIANTS), METHOD FOR PREPARING PROTEIN (VARIANTS), METHOD FOR SCREENING SUBSTANCE (VARIANTS), ANTIBODY (VARIANTS), METHOD FOR PREPARING POLYCLONAL ANTIBODY, HYBRIDOMA (VARIANTS), METHOD FOR PREPARING MONOCLONAL ANTIBODY, METHOD FOR ASSAY OF OCIF-BINDING PROTEIN, PHARMACEUTICAL COMPOSITION (VARIANTS) AND MEDICINAL AGENT (VARIANTS) Russian patent published in 2004 - IPC

FIELD: biotechnology, genetic engineering, medicine, molecular biology, pharmacy.

SUBSTANCE: invention can be used for preparing a protein binding specifically with osteoclastogenesis-inhibiting factor (OCIF). OCIF-binding protein binds specifically with osteoclasogenesis-inhibiting factor and elicits high affinity with respect to OCIF (dissociation constant on cellular membrane: K_d = 10^-9 M or less), its molecular mass is about 30 000-40 000 Da in measuring by electrophoresis in SDS-polyacrylamide gel under non-reducing conditions. Protein elicits activity for supporting and enhancing the differentiation and maturing osteoclast cells. Recombinant OCIF-binding protein is prepared by culturing cells transformed with DNA-containing vector encoding OCIF-binding protein. Antibody recognizing OCIF-binding protein is prepared by immunization of animal or by culturing hybridoma BP-6264 or BP-6265, or BP-6266. Antibody is used for preparing a pharmaceutical composition used for prophylaxis or treatment of disorder or pathology in osseous metabolism. Invention provides carrying out the development of agent used for prophylaxis or treatment of diseases associated with disturbances in osseous metabolism.

EFFECT: improved preparing method, valuable biological and medicinal properties of protein.

50 cl, 33 dwg, 4 tbl, 40 ex