STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS 22 AS PRODUCER OF SITE-SPECIFIC RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-CCNNNNN^NNGG-3' AND ELICITING GROWTH-STIMULATING ACTIVITY WITH RESPECT TO CULTURAL PLANT SEEDS Russian patent published in 2004 - IPC

Abstract RU 2238972 C2

FIELD: biotechnology, microbiology, biochemistry, agriculture.

SUBSTANCE: invention relates to the strain of bacterium Bacillus stearothermophilus 22 - a producer of site-specific restriction endonuclease recognizing and cleaving the nucleotide sequence 5'-CCNNNN^NNGG-3' and eliciting property of the growth-stimulating activity. Microorganism Bacillus stearothermophilus 22 is a producer of site-specific restriction endonuclease Bst 22I that is isolated from soil of hot springs in peninsula Kamchatka as result of search of producers of site-specific restriction endonuclease. Invention provides preparing site-specific restriction endonuclease recognizing and cleaving the nucleotide sequence 5'-CCNNNNN^NNGG-3'. The yield of enzyme is 50 000 U/g of wet biomass. Site-specific restriction endonuclease Bst 22I is an isoschizomer of restriction endonuclease Bsi YI and can replace the latter in all genetic engineering works. Biomass of cells of the bacterial strain elicits the growth-stimulating activity with respect to seeds of different cultural plants.

EFFECT: valuable biological properties of strain and restriction endonuclease.

3 tbl, 4 dwg, 6 ex

Similar patents RU2238972C2

Title	Year	Author	Number
STRAIN OF BACTERIUM Paenibacillus sp, PRODUCER OF RESTRICTION ENDONUCLEASE Psp 1009I	2007	Puchkova Larisa Ivanovna Andreeva Irina Sergeevna Saranina Irina Vasil'Evna Pechurkina Natal'Ja Ivanovna Afonina Veronika Sergeevna Repin Vladimir Evgen'Evich	RU2340663C1
STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-GGTNACC-3'	1996	Andreeva I.S. Puchkova L.I. Saranina I.V. Lokhova I.A. Repin V.E.	RU2115728C1
STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5′-CC(A/T)-GG-3′	1992	Repin Vladimir Evgen'Evich	RU2038380C1
STRAIN OF BACTERIUM BACILLUS SCHLEGELII - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5'-CCNNNNNNNGG-3'	1994	Repin V.E. Tereshchenko T.A. Lebedev L.R.	RU2073717C1
BACTERIUM STRAIN SPHINGOBACTERIUM MIZUTAE-32 AS PRODUCER OF SPML RESTRICTION ENDONUCLEASE, RECOGNIZING AND CLEAVING 5'-AT'CGAT-3' NUCLEOTIDE SEQUENCE	2003	Puchkova L.I. Radionenko Ju.V. Ushakova T.A. Repin V.E.	RU2266323C2
STRAIN OF BACTERIUM BACILLUS COAGULANS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5′-GATNNNN-ATC-3′	1994	Repin V.E. Tereshchenko T.A. Lebedev L.R.	RU2057806C1
STRAIN OF BACTERIUM BACILLUS SPECIES - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-(A/G)-CCGG-(T/C)-3'	1992	Repin Vladimir Evgen'Evich	RU2037522C1
STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF RESTRICTION ENDONUCLEASE ECO 75 KI	1993	Pertsev A.V. Kravets A.N. Beletskaja I.V. Zakharova M.V. Solonin A.S.	RU2053298C1
STRAIN OF BACTERIA BACILLUS COAGULANTS - PRODUCER OF RESTRICTION ENDONUCLEASE BCO AI	0	Sokolov Nikolaj Nikolaevich Anikejcheva Nina Vasilevna Eldarov Mikhail Anatolevich Fitsner Andrej Borisovich Karpychev Igor Viktorovich Kalugin Aleksej Aleksandrovich Samko Olga Tikhonovna Khoroshutina Ella Borisovna Librik Galina Isakovna	SU1761804A1
STRAIN OF BACTERIA BACILLUS ALVEI - PRODUCER OF RESTRICTION ENDONUCLEASE BAV BII	0	Sokolov Nikolaj Nikolaevich Anikejcheva Nina Vasilevna Fitsner Andrej Borisovich Samko Olga Tikhonovna Khoroshutina Ella Borisovna Kalugin Aleksej Aleksandrovich Librik Galina Isakovna	SU1761803A1

RU 2 238 972 C2

Authors

Puchkova L.I.

Andreeva I.S.

Ushakova T.A.

Radionenko Ju.V.

Repin V.E.

Polushkina A.F.

Dates

2004-10-27—Published

2002-06-05—Filed